Composite
Part:BBa_K539732:Design
Designed by: Yang, Yi-Pei Group: iGEM11_NCTU_Formosa (2011-09-24)
kivd (alpha-ketoisovalerate decarboxylase) with native rbs(BBa_B0030) and terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 599
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1676
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is an available coding sequence that can be placed at the end of the circuit. We also made a similar biobrick that includes the different rbs .
Source
Kivd is cloned form Lactococcus lactis subsp, lactis KF147, by Zeng lab ,NCTU-Formosa.